Fig. 3

Screening and analysis of the core target molecule S100A9. (A) Venny plot of the top 10 genes upregulated in the EAC group and 18 candidate key genes for IC/BPS. (B-C) Analysis of S100A9 expression in the bladders from EAC mice and IC/BPS patients. (D) ROC curve analysis of S100A9 gene expression in the ulcer and non-ulcer groups of patients with IC/BPS. (E) Immunohistochemical analysis of S100A9 expression in EAC mice (×200, n = 6). (F) Western blot analysis of S100A9 expression in EAC mice. (G) Immunohistochemical analysis of S100A9 expression in IC/BPS patients (×200, n = 6). NS indicates no difference; *p < 0.05, **p < 0.01, ***p < 0.001