Fig. 5

Expression analysis of S100A9 in EAC group from data of single-cell sequencing. (A) Venny plot of overlapping genes related to S100A9 and S100A8 comparing within the EAC and control groups. (B) GO enrichment analysis of gene pathways associated with S100A9 and S100A8. (C) Distribution and expression analysis of S100A9 and S100A8 in different bladder cell types between control and EAC group. (D) Comparative analysis of S100A9 and S100A8 expression in macrophages between control and EAC groups. (E) Expression analysis of TLR4 receptor in bladder cells between control and EAC groups. (F) Comparative analysis of gene expression in macrophages between the EAC and control groups. (G) GSEA analysis of the activation pathway of MACROPHAGE ACTIVATION. (H) Analysis of cell-cell communication source of neutrophils between control and EAC group. (I) Analysis of cell-cell communication source of macrophages between control and EAC group. (J) Immunofluorescence confocal analysis of S100A9 and macrophage marker F4/80 in control and EAC groups (n = 6)