Fig. 8

Analysis of the effects of S100A9 knockdown on bladder tissue and immune cells in EAC mice (n = 6). (A) HE staining analysis of bladder tissues in WT and S100A9 knockdout mice of control and EAC groups (×200). (B) Analysis of mast cell infiltration in WT and S100A9 knockout bladder tissues from control and EAC groups (×200). (C) TUNEL staining for apoptosis analysis in bladder tissues from WT and S100A9 knockout mice in the control and EAC groups (×200). (D) Immunohistochemical analysis of macrophage marker F4/80 in WT and S100A9 knockout mice of control and EAC groups (×200). (E) Immunohistochemical analysis of CD4 in WT and S100A9 knockout mice of control and EAC groups (×200). (F) Immunohistochemical analysis of S100A9 in WT and S100A9 knockdout mice of the control and EAC groups (×200). NS indicates no difference; *p < 0.05, **p < 0.01, ***p < 0.001