Fig. 2

Identification of NKt-AML candidate antigens. A Dot plots show candidate antigen protein intensity in NKt-AML samples from the Leucegene AML surfaceome cohort. P values were calculated using the Wilcoxon test. B Single cell RNA expression of candidate antigens along with ADGRG1 in all cells (left) and CD34⁺ HSC like cells (right) from NKt-AML samples and normal BM (data from the Human cell atlas). The percentage of gene-expressing cells along with Z-scores calculated per gene for expression in the different samples are shown. Barplots on top represent the number of cells per sample. C UMAP representation of single-cell RNA-sequencing data from NKt-AML samples and normal BM colored according to sample identity (top) or to expression of candidate antigens as well as ADGRG1 (bottom). D Log2 FC expression ratio of each candidate in CD34 + HSC like cells of NKt-AML vs BM (grey bars) or individual sample vs BM (colored bars, same color code). Antigens in the left panel are selected according to the pipeline. Antigens in the right panel are FDA approved (or clinical Phase I completed) targets, that are selectively expressed on NKt-AML (don’t match selection pipeline)